CONTROL OF BACTERIAL CONTAMINATION OF DENTAL UNIT WATER USING SODIUM HYPOCHLORITE
Resumo
Objective: To evaluate bacterial load of water using and not using sodium hypochlorite aiming at bacterial control of dental unit water. Method: Dental unit reservoirs from a dental clinic from School of Dentistry of Ribeirão Preto -USP were filled with public-supply water with and without sodium hypochlorite solution at 0.0003%, and water samples were collected and evaluated by Petrifilm™ AC (Aerobic Count) system from 2014 to 2017. Results: No bacterial contamination was observed in the water samples using sodium hypochlorite solution. However, bacterial contamination was detected in water from reservoirs, air-water syringes and high-speed outputs (without handpieces) without the disinfectant. Conclusion: The use of sodium hypochlorite solution controlled bacterial load of all the dental unit water samples as established by Brazilian legislation (<500CFU/mL).
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Referências
Aas JA, Paster BJ, Stokes LN, Olsen I, Dewhirst FE. Defining the normal bacterial flora of the oral cavity. J Clin Microbiol [Internet]. 2005 Nov [cited 2019 Jun 05];43(11):5721-5732. Available from: http://jcm.asm.org/content/43/11/5721.abstract
Ito IY, Souza-Gugelmin MCM, Lima SNM. Assepsia e anti-sepsia em endodontia. In: Leonardo, MR, Leal JM. Endodontia: tratamento de canais radiculares. 3rd ed. São Paulo: Médica Panamericana; 1998. p. 261-297.
Mills SE, Karpay RI. Dental waterlines and biofilm—searching for solutions. Compend Contin Educ Dent [Internet]. 2002 Mar [cited 2019 Jun 05];23(3):237-258. Available from: https://www.ncbi.nlm.nih.gov/pubmed/12785138
Miller CH, Palenik CJ. Infection control and management of hazardous materials for the dental team. 2nd ed. St. Louis: Mosby; 1998. Dental unit water asepsis; p. 190-204.
Brasil. Ministério da Saúde. Portaria no 2.914 de 12 de dezembro de 2011. Dispõe sobre os procedimentos de controle e de vigilância da qualidade da água para consumo humano e seu padrão de potabilidade. Diário Oficial da União, Brasília, 14 dez. 2011. Available from: http://bvsms.saude.gov.br/bvs/saudelegis/gm/2011/prt2914_12_12_2011.html
Michałkiewicz M, Ginter-Kramarczyk D, Kruszelnicka IK. Is water in dental units microbiologically safe? Med Pr [Internet]. 2015 [cited 2019 Jun 05];66(6):763-70. Available from: http://medpr.imp.lodz.pl/Is-water-in-dental-units-microbiologically-safe-,58316,0,2.html
Ji XY, Fei CN, Zhang Y, Zhang W, Liu J, Dong J. Evaluation of bacterial contamination of dental unit waterlines and use of a newly designed measurement device to assess retraction of a dental chair unit. Int Dent J [Internet]. 2016 Aug [cited 2019 Jun 05];66(4):208-214. Available from: https://onlinelibrary.wiley.com/doi/full/10.1111/idj.12225
Blake GC. The incidence and control of bacterial infection in dental spray reservoirs. Br Dent J [Internet]. 1963 [cited 2019 Jun 05];115(10):413-416. Available from: https://www.cabdirect.org/cabdirect/abstract/19642702287
Kelstrup J, Funder-Nielsen TD, Theilade J. Microbial aggregate contamination of water lines in dental equipment and its control. Acta Pathol Microbiol Scand B [Internet]. 1977 Jun [cited 2019 Jun 05];85(3):177-183. Available from: https://www.ncbi.nlm.nih.gov/pubmed/329637
American Dental Association. ADA statement on dental unit waterlines. J Am Dent Assoc [Internet]. 1996 Feb [cited 2019 Jun 05];127(2):185-186. Available from: https://jada.ada.org/article/S0002-8177(15)60463-9/pdf
Centers for Disease Control and Prevention (CDC). Recommended Infection-Control Practices for Dentistry, 1993. MMWR Recomm Rep [Internet]. 1993 May [cited 2019 Jun 05]; 42(RR-8):1-12. Available from: https://www.cdc.gov/mmwr/preview/mmwrhtml/00021095.htm
Monteiro RM, Marques DM, Domingues PCA, Oliveira VC, Macedo AP, Razaboni AM, et al. Evaluation of a protocol for reducing the microbial contamination of dental unit water. Acta Odontol Latinoam [Internet]. 2018 Dec [cited 2019 Jun 05];31(3):138-143. Available from: http://www.scielo.org.ar/pdf/aol/v31n3/v31n3a03.pdf
Ricci ML, Fontana S, Pinci F, Fiumana E, Pedna MF, Farolfi P, et al. Pneumonia associated with a dental unit waterline. Lancet [Internet]. 2012 Feb [cited 2019 Jun 05];379(9816):684. Available from: https://www.sciencedirect.com/science/article/pii/S0140673612600749?via%3Dihub
Lal S, Singhrao SK, Achilles-Day UE, Morton LH, Pearce M, Crean S. Risk assessment for the spread of Serratia marcescens within dental-unit waterline systems using Vermamoeba vermiformis. Curr Microbiol [Internet]. 2015 Oct [cited 2019 Jun 05];71(4):434-442. Available from: https://link.springer.com/article/10.1007%2Fs00284-015-0872-0
Schönning C, Jernberg C, Klingenberg D, Andersson S, Pääjärvi A, Alm E, et al. Legionellosis acquired through a dental unit: a case study. J Hosp Infect [Internet]. 2017 May [cited 2019 Jun 05];96(1):89-92. Available from: https://www.sciencedirect.com/science/article/pii/S0195670117300154?via%3Dihub
Silva AS, Ribeiro MC, Risso M. Biossegurança em Odontologia e ambientes de saúde. 2nd ed. rev. ampl. São Paulo: Ícone; 2009. Controle da qualidade da água; p. 169-173.
Karpay RI, Plamondon TJ, Mills SE, Dove SB. Combining periodic and continuous sodium hypochlorite treatment to control biofilms in dental unit water systems. J Am Dent Assoc [Internet]. 1999 July [cited 2019 Jun 05];130(7):957-965. Available from: https://doi.org/10.14219/jada.archive.1999.0336
O'Donnell MJ, Boyle M, Swan J, Russell RJ, Coleman DC. A centralised, automated dental hospital water quality and biofilm management system using neutral Ecasol maintains dental unit waterline output at better than potable quality: a 2-year longitudinal study. J Dent [Internet]. 2009 Oct [cited 2019 Jun 05];37(10):748-762. Available from: https://doi.org/10.1016/j.jdent.2009.06.001
Porteous N, Schoolfield J, Luo J, Sun Y. The biofilm-controlling functions of rechargeable antimicrobial N-halamine dental unit waterline tubing. J Clin Dent [Internet]. 2011 Jan [cited 2019 Jun 05];22(5):163–170. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4450763
Petti S, Moroni C, Messano GA, Polimeni A. Detection of oral streptococci in dental unit water lines after therapy with air turbine handpiece: biological fluid retraction more frequent than expected. Future Microbiol [Internet]. 2013 Mar [cited 2019 Jun 05];8(3):413-421. Available from: https://doi.org/10.2217/fmb.12.151
Lizzadro J, Mazzotta M, Girolamini L, Dormi A, Pellati T, Cristino S. Comparison between Two Types of Dental Unit Waterlines: How Evaluation of Microbiological Contamination Can Support Risk Containment. Int J Environ Res Public Health [Internet]. 2019 Jan 24 [cited 2019 Jun 05];16(3):328. Available from: https://dx.doi.org/10.3390%2Fijerph16030328
DOI: https://doi.org/10.26694/repis.v5i0.8502
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